| # | Player | Position | Class |
|---|---|---|---|
| 1 | Seth Rushford | DB, WR | |
| 2 | Tommy Rhomberg | DB, QB | |
| 3 | Michael Ryan | - | |
| 4 | Landon Wilson | DB, WR | |
| 5 | Drew Thurn | DB, WR | |
| 5 | Graham Yanda | LB, QB | - |
| 6 | Trae Bielefeld | DB, QB | |
| 7 | Brendan McNeal | DB, K, WR | - |
| 8 | Kellen Haverback | DB, QB | |
| 9 | Jase Jaspers | LB, RB | |
| 10 | Easton Reasland | DB, QB | |
| 11 | Maddox Curran | DB, WR | |
| 13 | Stanley Krob | DB, WR | |
| 15 | Jackson Krug | CB, DB, WR | |
| 16 | Toby Neal | - | |
| 18 | William Goodlove | - | |
| 19 | Holden Hlavacek | S, WR | |
| 20 | Luke Robinson | LB, RB | - |
| 21 | Logan Stanerson | DB, WR | - |
| 22 | Jakob Yock | ILB, LB, P, RB | |
| 23 | Xander Wegner | OLB, WR | |
| 24 | Jack Carnes | K, LB, P | |
| 25 | Gabe Grafft | LB, RB | - |
| 26 | Teddy Bonewald | CB, DB, WR | |
| 30 | Caleb Keegan | DB, WR | |
| 31 | Caysen Curran | ILB, RB | |
| 32 | Ian Hagen | DB, WR | |
| 33 | Ryan Hepker | DB, WR | |
| 34 | Jake Shady | LB, RB | |
| 38 | Logan Ericson | DB, WR | |
| 50 | Dylan Marting | DL, OL | - |
| 51 | Jakob DeLany | H, LB, OL | |
| 52 | Cole Weddington | DL, OL | |
| 53 | Andrew Dix | LB, OL, OLB | |
| 54 | Jaxon Anderson | ILB, TE | |
| 55 | Leuie Korte, II | - | |
| 56 | Ryker Lee | DL, OL | - |
| 57 | Xander Riniker | ILB, LB, OL, RB | |
| 58 | Cameron Poggenpohl | DL, OL | |
| 60 | Owen Wheat | DL, OL | |
| 61 | Eli Plotz | DL, OL | |
| 62 | Jackson Koehn | DL, OL | - |
| 63 | Gunner Steinberg | DL, LB, OL | |
| 64 | Ryan Hoffman | ||
| 65 | Holden Steine | DL, OL | |
| 66 | Zach Broomhall | LB, OL | |
| 69 | Alex Briesemeister | - | |
| 70 | Brogan Meyer | DL, OL | |
| 72 | Landen Conley | DL, OL | - |
| 74 | Andrew Errington | DL, OL | - |
| 75 | Brady Weaver | DL, OL | |
| 76 | Landyn Leyse | DL, OL | |
| 77 | Beckett Curran | DL, OL | - |
| 78 | Hutson Wiesenhofer | DL, OL | |
| 79 | Hayden Gail | DL, OL | - |
| 80 | Matthew Goodlove | DB, RB | |
| 85 | Daxton Recker | DB, LB, WR | |
| 88 | Rowan Jensen | DB, DL, WR | |
| 89 | Kolter Flockhart | LB, WR |
